Tagfinder for the quantitative analysis of gas chromatography mass spectrometry gcmsbased metabolite profiling experiments alexander luedemann department prof. Gas chromatographymass spectrometry gc ms is a robust approach for the qualitative and quantitative analysis of metabolites of plant origin iordache et al. Pueraria tuberosa, methanolic extract, gcms analysis, bioactive compounds. Melastomastrum capitatum is a plant use in mambila plateau in taraba state, nigeria for. This study forms a basis for the biological characterization and importance of the compounds identified and. Gcms analysis of bioactive compounds from the whole plant. Gcms chromatogram analysis of the ethanolic extract of p.
The extracts were concentrated by evaporation and subjected to freeze drying till dry powder was. The methanol fractions of each plant were taken for gc ms analysis. Pdf characterization of some plant extracts by gcms. A total of 81 compounds were identified by gas chromatography mass spectrometry. Gas chromatographymass spectrometry gcms analysis of the whole plant methanol extract of lactuca runcinata was performed on a gcms equipment. The bioreduction activity of plant extract is higher than that of. The component identification was achieved by the gcms analysis using hp 6890 series gc hewlettpackard. Gcms analysis of the essential oil and methanol extract. The present experiment was designed to determine the bioactive constituents. The tubes were uniformly illuminated with an incandescent visible light for 15 minutes, and the optical. The gcms system consisted of an as 2000 autosampler, a gc 8000 gas chromatograph and a voyager quadrupole mass spectrometer thermoquest, manchester, uk. Gricilda shoba abstract justicia adhatoda leaf extracts were prepared in methanolic solvent to study the phytochemical profile using gas chromatography mass spectrometry.
Research article gcms and lcms analysis of kakadu plum fruit. Extraction and gcms analysis of the human blood plasma. Macrotyloma uniflorum linn fabaceae is a herbaceous plant with annual branches. The ethanolic extracts of the plant was used for gcms analysis.
To conduct a gcms analysis on ethanolic leaves extracts 25 com. Gcms analysis of these extracts was carried out by following the method of hema et al. It is used in kidney stones, inflamed joints, fever, musculoskeletal disorders, sinus wounds and localized. Chemical composition of ethanol extract of macrotyloma. To identify the various phyto constituents present in the unexplored plant rostellularia diffusa willd. The results pertaining to gcms analysis led to the identification of number of terpenes from hexane. A fast gc application using high resolution gcms for the european priority. Gcms analysis revealed the presence of the following phytocompounds. The investigation was carried out to determine the possible bioactive components of the stem bark of pteleopsis suberosa using gcms analysis. Ms analysis, the sfe extract often requires additional cleaning steps to remove fats or pig. The tlc results indicated that four 4 terpenes are present in hexane extracts of the leaf of syzygium guineense after treating tlc plates with vanillinconc.
Gcms analysis of terpenes from hexane extract of lantana. Preliminary phytochemical screening and gc ms issn. Gcms analysis of bioactive compounds present in different. Gcms analysis, phyllodium pulchellum, ethanol leaf extract, bioactive. Gcms analysis of phytocomponents in the various extracts. The aim of this study was to carry out for identification of bioactive compounds from the whole plant ethanolic extract of evolvulus alsinoides by gas chromatography and mass spectroscopy. Gas chromatography mass spectrometry gcms is a robust approach for the qualitative and quantitative analysis of metabolites of plant origin iordache et al. Ftir analysis infra red spectral analysis was performed using shimadzu ftir 8300 instrument. The results pertaining to gcms analysis leads to the identification of number of compounds from the gc fractions of the methanolic extract of trichosanthes anguina l. Phytochemical analysis by gcms analysis of the plant rhizomes revealed the presence of different fatty acids, heterocyclic compounds etc. Plants have more vital sources of natural products and involving in the human health care system since the human civilization starting.
Analysis of the entire set of low molecular weight compounds lmc, the metabolome, could provide deeper insights into mechanisms of disease and novel markers for diagnosis. Qualitative phytochemical screening and gcms analysis of. Soxhlet extraction is a solubilitybased extraction method for solid samples. Gcms analysis of pteleopsis suberosa stem bark methanol. Gcms analysis of bioactive compounds in methanolic leaf extracts of justicia adhatoda linn. The presence of 3omethyl dglucose by gcms analysis in both a. Gcms analysis of essential oil of pogostemon cablin growing in indonesia extracted by microwaveassisted hydrodistillation abstract the essential oil from the leaf of pogostemon cablin, grown in indonesia was extracted by microwaveassisted hydrodistillation and analyzed by gas chromatography mass spectrometry. In order to obtain the information of the kinds of compounds in methanol extract of bark of tampoi, an analysis using gcms 5977 was performed. Gcms analysis of various crude organic extracts of m. The crude extract form was subjected to column chromatography over silica gel 100200 mesh and eluted with nhexane, chloroform, ethanol and methanol respectively. Tlc analysis and gcms profiling of hexane extract of s. Gcms analysis of ethyl acetate extract of whole plant of. Gcms analysis of stem bark extracts of senna alata l. Active extracts were analysed by nontargeted hplcqtof mass spectroscopy.
Gcms analysis of phytocomponents in the ethanol extract. Gcms analysis of ethanolic extract was done by standard protocol using the equipment thermo gctrace ultra version. The gcms analysis of volatile components extracted by. Gcms analysis for structural identification and bioactive. Gcms analysis of the whole plant extract of evolvulus alsinoides was performed using the equipment thermo gc. Gcms analysis of bioactive compounds from melastomastrum. Gcms is the best technique to identify the bioactive components of long chain hydrocarbons, alcohols, acids used in the analysis of the herbal medicines, there are more significant advantages for gcms. Gas chromatography mass spectrometric gcms and quantitative. These phytocompounds may have attributed to the antimicrobial potential of the plant. Identification of phytochemicals through gc ms analysis gcms technique. The sample was introduced via an allglass injector working in the split mode, with helium carrier gas low rate of 1.
Gcms analysis were performed using a perkinelmer gc clauses 500 system and gas chromatograph. Gcms analysis of essential oil of pogostemon cablin. Gcms analysis and phytochemical screening of a rare. Kbr pellet was prepared by mixing 1mg of fungus extract with 10mg of anhydrous potassium bromide. The gcms analysis of the ethanolic extract revealed the presence of 14 major compounds. The gcms analysis was performed by using perkin elmer clarus 500 model with the software turbomass ver 5. Pdf gcms analysis of bioactive compounds on ethanolic leaf. Gcms analysis of bioactive phytochemicals present in ethanolic. Antimicrobial activity of all plant extracts was determined using a modified disc.
Pdf phytochemical, gcms analysis and antibacterial. Gcms analysis the essential oil and the crude methanol extracts of the seeds were analysed in southampton, england on a capillary gc column and the results were presented with 5%, 1% and 0. The chemical and surface analysis of the synthesized file silver nanoparticles. The component identification was achieved by the gc ms analysis using hp 6890. The obtained extracts were stored then subjected to further analysis. The gc ms analysis of bioactive compounds from the different extracts of the leaves of b. The sample comprised 99% pure hemp extract in a base of hempseed oil, unspiked and spiked at 10 ngg with the solvents listed in table 1. Volatile compound analysis using gcms, phytochemical. The whole plant of rostellularia diffusa was extracted with ethyl acetate at room temperature for 72 h. Evaluation of antimicrobial activity and gcms profiling. Hplc and gc analysis uhplc analysis was carried out using agilent 1290. The detailed tabulations of gc ms analysis of the extracts are given in table 1, table 2, table 3, table 4 and table 5 respectively. Gcms analysis of secondary metabolites of endophytic. Gcms analysis of phytocomponents in the methanolic extract.
Gcms analysis revealed the presence of hexadecanoic acids and their. The extraction method presented is simple, rapid and inexpensive, with reduced solvent. Gas chromatographymass spectroscopic analysis of evolvulus alsinoides. The concentrated extract was subjected to gcms analysis to detect the phyto constituents. Gcms analysis of phytocomponents in methanolic extracts. The gcms analysis shows different peaks with low and high molecular weight.