Qiagen kit handbooks and user manuals are available at. Rneasy plus kit high performance rna extraction youtube. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user manual. Qiagen qiaamp dna micro kit, 50 preps, 1 to 100l sample, 20 to 100l elution volume, whole. Total rna and mirna can also be copurified from formalinfixed, paraffinembedded.
Qiagen clinical kits compatible with the qiavac 24 plus application kit accessories genomic and viral dna purification from blood and body fluids qiaamp dna blood kits vacconnectors and vacvalves qiaamp dna mini kit vacconnectors and vacvalves viral rna purification from plasma. Qiagen genomictips are available for purification of up to 500 g of genomic dna from blood, cultured cells, tissue, yeast, and gramnegative bacteria. Rna purification kits do not recover rna molecules smaller than 200 nucleotides. Qiagen typeit microsatellite pcr kit 70 laboratory. Jan 22, 2018 using builtin gdna eliminator technology, the rneasy plus kit gives you high yields of highintegrity rna in minutes for any downstream application, without the need for dnase. Rna isolation protocol using the qiagen rneasy mini kit materials and preparation of solutions. Item qiaamp dna investigator kit 50 company qiagen. Rneasy mini handbook rneasy mini kit for purification of total rna from animal cells, animal tissues, bacteria, and yeast, and for rna cleanup rneasy protect mini kit for immediate stabilization of rna in harvested animal tissues and subsequent total rna purification rneasy plant mini kit for purification of total rna from plants and filamentous fungi fourth edition june. I am looking for a good procedure for high rna yields that would be compatible with micro array. The qiaamp dna micro kit uses wellestablished technology. For reverse transcription of 50 ng to 2 micro g rna per reaction for endpoint pcr. The qiaamp dna micro kit simplifies purification of genomic and mitochondrial dna with a fast spincolumn procedure. Small numbers of sorted cells, cultured cells and tissues may be disrupted in qiagen rlt buffer purified using qiagen rneasy micro kit. The qiaamp dna investigator kit provides purification of genomic dna from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters.
Rlt sample lysis buffer ethanol 70% and 80% rw1 washing buffer rdd buffer store in fridge dnase in rdd buffer rpe rnase free water add 10l mercaptoethanol1ml rlt rlt is stable for a moth at rt after addition of mercaptoethanol. The typeit microsatellite pcr kit is based on highly specific hotstartaq plus dna polymerase and a patented buffer system, both of which enable reliable multiplex pcrbased microsatellite analysis without optimization. The rneasy micro kit is shipped at room temperature 1525c. Qiagen rneasy micro protocol for sorted cells note. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf, blood, body fluids, or washed ce. The rneasy ucp micro kit is shipped at ambient temperature. For total rna isolation from a wide variety of sources. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. Qiaquick spin kits are developed, designed and sold for research purposes only. Both the qiaprep spin miniprep kits and qiagen plasmid plus kits work with the manifold, which can handle up to 24. For simultaneous purification of dna and rna from cells and tissues highquality dna and rna from the same sample maximal yields of dna and rna from precious samples rapid purification with short, streamlined protocol readytouse dna and rna for any downstream analysis the allprep dnarna mini kit. Tissuelyser handbook university of texas at arlington.
Qiaamp dna mini kit bacterial or genomic dna purification from water, filters, or surface swabs by qiagen. This protocol is adapted from the qiagen rneasy micro handbook, 2007. Lyophilized qiagen protease can be stored at room temperature 1525c for up to. The handbook gives enough variables that you can apply it to your specific needs. Qiagen plasmid purification handbook 082003 9 product use limitations qiagen, qiafilter, and endofree plasmid kits are developed, designed and sold for research purposes only. Using builtin gdna eliminator technology, the rneasy plus kit gives you high yields of highintegrity rna in minutes for any downstream application, without the need for dnase. In accordance with qiagens isocertified quality management system, each lot of the qiaamp dna mini kit and the qiaamp dna blood mini kit is tested against predetermined specifications to ensure consistent product quality. Qiagen dramatically ramping up global production capacity for rna extraction kits. Business strategy, stock and financial performance. For highthroughput purification in a 96well format, the mirneasy 96 kit is available.
The qiaamp dna micro kit uses wellestablished technology for purification of genomic and mitochondrial dna from small sample volumes or sizes. Rlt sample lysis buffer ethanol 70% and 80% rw1 washing buffer rdd buffer store in fridge dnase in rdd buffer rpe rnase free water. For highthroughput purification in a 96well format, the mirneasy 96 kit. Qiagen s rneasy mini kit and rneasy plus micro kit having low yields of rna mar252009 i tried using the rneasy plus micro kit claims it can isolate rna from a single cell at first to extract rna from 20,000 cells human es cells but i only got a very low yield of 22 ngul 14 ul elution volume. Fda eua for qiastatdx test kit, first and only syndromic solution integrating detection of sarscov2 coronavirus. Qiagen clinical kits compatible with the qiavac 24 plus application kit accessories genomic and viral dna purification from blood and body fluids qiaamp dna blood kits vacconnectors and vacvalves qiaamp dna mini kit. Qiaamp dna mini kit nucleic acid purification bacterial. Rnasefree dnase set box, containing rnasefree dnase, buffer rdd, and rnasefree water, should be stored immediately upon receipt at 28c. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. Qiagen rneasy micro kit, 50 preps, 10 to 14l elution volume, kit content. No claim or representation is intended for their use to identify any specific. We get really good results using the qiagen dna kits, with high dna yields, which can be used for sequencing.
Rneasy minelute cleanup handbook harvard university. Qiagen plasmid purification handbook harvard university. The purchaser must determine the suitability of the product for its particular use. Jun 25, 2015 rna extraction from plants using the rneasy plant mini kit. The following risk and safety phrases apply to components of the rneasy mini kit, the. Qiaprep spin midimaxiprep kit protocol before staring make sure that rnase a has been added to p1 buffer. We recommend all users of qiagen products to adhere to the nih guidelines that have been developed for recombinant dna experiments, or to other applicable guidelines. Print bookmark share for purification of total rna from small samples, including. The highly pure dna prepared with qiagen genomictips is free of all contaminants and ranges in size up to 150 kb.
The rneasy micro kit is shipped at ambient temperature. Qiaseq mirna library kit handbook, illumina ngs systems 122018 2 contents. The remaining components of the rneasy micro kit should be stored dry at room temperature. Should any product fail to perform satisfactorily due to any reason other. For purification of genomic and mitochondrial dna from small samples. Qiagen mirneasy micro kit, 50 preps, easy, robust procedures, automatable protocols, for purification of mirna and total rna from small amounts of cells and tissues, highpurity rna suitable, ideal for northern blot analysis, quantitative, realtime rtpcr, microarray analysis. Total rna isolation with rneasy micro qiagen spin protocol. Qiaamp dna investigator kit 50 56504 from qiagen sample. A variety of microorganisms, including bacterial and fungal spores, have been tested successfully with this kit.
No claim or representation is intended for their use to identify any specific organism or for a specific clinical use diagnostic, prognostic, therapeutic, or blood banking. I want to use the qiagen rneasy micro plus kit on 4 crab hemolymph pellet samples. Qiaamp genomic dna kits 122015 7 qiaamp dna micro kit purification of genomic and mitochondrial dna from small samples the qiaamp dna micro kit is designed for purification of genomic, mitochondrial and viral dna from small amounts of fresh or frozen blood, tissue and dried blood spots. The kit uses qiaamp minelute spin columns for purification of highquality dna with flexible elution volumes. Hannah wardill, biomedical research fellow, university of groningen and university. Our technical service departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of qiagen. Minelute spin columns and the rnasefree dnase set i. The rneasy micro kit is designed for purification of up to 45. Qiagen qiaamp dna micro kit, 50 preps, 1 to 100l sample, 20 to 100l elution volume, whole blood sample, silica technology, manual processing, spin column format, genomic dna, mitochondrial dna purification, 30 min. The mirneasy micro kit is designed for purification from small amounts of cultured cells, sorted cells, laser capture microdissection samples from cryosections, and. Rneasy mini handbook 042006 7 the rneasy mini kit and rneasy protect mini kit are intended as generalpurpose devices.
Rneasyminikit for purification of total rna from animal cells, animal tissues, and yeast, and for cleanup of rna from. Rneasy micro handbook 042003 5 all due care and attention should be exercised in the handling of the products. The handbook supplied with your qiasymphony important kit will tell you which protocol you should use. They are not to be used for human diagnostic or drug purposes or to be administered to humans unless expressly cleared for that purpose by the food and drug administration in the usa. Total rna is then purified using rneasy mini spin columns.
This offer is valid until december 31, 2019 and only when order is placed online through qiagen web shop, your organizations purchasing system, your sales rep, or by email using the cyber19 promo code. Alternative prefilled bead tube options are available for additional applications. Qiagen rneasy micro kit, 50 preps, 10 to 14l elution volume, kit for isolation of total rna from qiagen is a very convenient kit for isolation of good quality rna in a relatively short time. Qiagen launches qiastatdx test kit for detection of sarscov2 coronavirus in europe following ce marking. Store the rneasy minelute spin columns and the rnasefree dnase set i. In accordance with qiagens isocertified quality management system, each lot of the rneasy mini kit, rneasy protect mini kit, and rneasy plant mini kit is tested against predetermined specifications to ensure consistent product quality. For purification of mirna and total rna from small amounts of cells and tissues. The rneasy mini handbook provides protocols for use with the following kits. Dneasy powerplant pro kit handbook 052017 7 this kit provides powerbead tubes prefilled with 2. Print bookmark share for purification of total rna from small. This highly optimized solution facilitates both enhanced differential expression analysis using integrated unique molecular indices umis.
The kit combines the selective binding properties of a silicabased membrane with flexible elution volumes of between 20 and 100 l. The following risk and safety phrases apply to components of the rneasy mini kit, the rneasy protect mini kit, andor the rneasy plant mini kit. Qiagen qiaamp dna micro kit 50 laboratory consumables. Please contact qiagen technical service at support. Qiagen onestep rtpcr kit handbook 052002 5 technical assistance at qiagen we pride ourselves on the quality and availability of our technical support. The qiaamp dna micro kit simplifies isolation of dna from small samples with a fast spincolumn procedure see flowchart procedure. Total rna isolation with rneasy micro qiagen karine. Kits for rna purification from animal or human tissues using spin columns sample type kit kit format page rneasy micro kit up to 5 mg tissue.
Qiaamp dna micro handbook 082003 7 introduction the qiaamp dna micro kit uses wellestablished technology for purification of genomic and mitochondrial dna from small sample volumes or sizes. For purification of microrna and total rna from tissues and cells. Rneasy minelute cleanup handbook 102010 5 product warranty and satisfaction guarantee qiagen guarantees the performance of all products in the manner described in our product literature. Rneasy plus micro kit protocol using rna carrier and supernatant. Rneasy minelute cleanup handbook 032003 5 product warranty and satisfaction guarantee qiagen guarantees the performance of all products in the manner described in our product literature. This post contains a slightly modified rneasy kit protocol. Cell or tissue lysates are spun through gdna eliminator spin columns to remove genomic dna. The kit contains optimized components that allow both reverse transcription and pcr amplification to take place in what is commonly referred to as a onestep reaction. Print bookmark share for purification of total rna from small samples, including animal and human cells. For purification of up to 45 g total rna from cell and tissue samples. This offer is valid only from march 19 to march 29, 2018, and only when an order is placed online through the qiagen webshop using the promo code spring2018. Qiaamp dna investigator kit 50 56504 from qiagen sample to insight. Our technical service departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of qiagen prod ucts.